For my own info,
Does microED encompass work with 2D crystals, or only micro-3D crystals?
On 08/15/2020 10:40 PM, Jessica Bruhn wrote:
Hi Alex,
Welcome to the field of microED! From a practical standpoint, microED also
suffers from the phase problem, and somewhat moreso compared to X-ray
Ethan,
Thank you for describing existence of two types of polarizers. I use two
crystal imagers/incubators, a smaller machine is easy to adjust to compensate
for birefringence of plastic plates/covers, another is more capricious and the
compensation is uneven over a plate. Company engineers
On Sunday, 16 August 2020 12:14:59 PDT Diana Tomchick wrote:
> If only glass is placed between the polarizer and analyzer, the crystal will
> not show artificial colors (try it in a 9-well Pyrex depression plate). The
> artificial colors come from the diffraction of visible light from the
If only glass is placed between the polarizer and analyzer, the crystal will
not show artificial colors (try it in a 9-well Pyrex depression plate). The
artificial colors come from the diffraction of visible light from the plastic
ware, which depending upon the type of plastic and the way the
Hi All,
Adding some more details to what's been said already. Only because I've
seen too many times the polarizers being used incorrectly.
First, you need two polarization filters which are typically called
polarizer and analyzer. First one (the polarizer) lets through only the
light waves of a
You need to have crystal between polarizing plates. If you use only one on the
top (close to your eye) and rotate filter you will “filter/polarize” all the
light coming to your eye. What you need to buy is polarizing filter sheet
(linear) which you place under your crystal and another one (the
Hi, place a linear polarizer below the tray and one above it, rotating
the top one until you get the desired view.
V. Nagarajan
JANSi
On 8/16/2020 8:26 AM, Diana Tomchick wrote:
It's my understanding that you have two polarizers on your
polarizer-microscope--one in the base, and the one
It's my understanding that you have two polarizers on your
polarizer-microscope--one in the base, and the one that attaches to the
magnifying lens. When you rotate the one on the lens so that it is 90 degrees
to the one in the base, no (or very little) light should pass through to your
eyes,
Hi all,
The polarizer-microscope in our facility is not working properly, and I have
to check my plates using a standard stereo-microscope. As a workaround, I
thought about buying one at Amazon, placing it on top of the plates and
rotating it to still test for birefringence.
The product is
Greetings everyone,
I am looking for an assay suitable to detect atleast, 500nM to 1mM ATP
hydrolysis. My Kinase has a very weak activity. The signal should be good
enough (also must be duplicated) to have good signal/noise and Z´values.
I want to use the assay for Inhibitor screening. Then the
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