I have run ABACAS on my sequences and the following output files was the result:
6732_2#8_refH10407.bin 6732_2#8_refH10407.crunch 6732_2#8_refH10407.fasta 6732_2#8_refH10407.gaps 6732_2#8_refH10407.gaps.stats 6732_2#8_refH10407.gaps.tab 6732_2#8_refH10407.tab I want to compare the sequence to my reference. In what place would I put which files? See below: ACT: Sequence 1: Comparison file: Sequence 2: I also want to view my sequence against another gene sequence. Correct me if I am wrong, I would need to run a blast and then use the output comparison file in ACT? Which blast script would one prefer? I have hear of blastall, blast by MLST but I don't know why to choose either of them. Thanks in advance! Regards, Astrid --- Astrid von Mentzer, PhD-student University of Gothenburg Institute of Biomedicine Department of Microbiology and Immunology Box 435 405 30 Göteborg Sweden Phone: +46 31 786 62 21 Fax: +46 31 786 62 05
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