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Hey Mousheng, Have you checked your data carefully for twinning?? I am a new graduate student and dont have alot of experience but from what I understand certain forms of twinning can go undetected during structure solution but can hinder your refinement.... hope this helps. Sean On Fri, 25 Aug 2006 18:51:40 +0800 "Wu Mousheng" <[EMAIL PROTECTED]> wrote: > > > Dear all, > > > > I am refining my model with 2.0A using CNS. I have fitted most of the > protein sequence into the model (150 of 160 aa). I also added 100 > water > molecules into the model. The final R and Rfree factor are about 26% > and > 28%, respectively. I am wondering why I can not refine my model to > low R > and Rfree factors below 25%. The geometry of my final model is very > good. 96% residues are in most favorable region. > > > > I think my data is quite good. I/sigma(unaverage) and completeness at > highest resolution shell is 2.3 and 99%. Rmerge is about 6%. > Redundancy > is about 5. > > > > Is my model acceptable based on the R and Rfree factors? Is there any > reason for the high R and Rfree factors? > > > > Thanks! > > > > Best, > > Mousheng Wu, > > Institute of Molecular and Cell Biology, Singapore > > > > > > DISCLAIMER: > This email is confidential and may be privileged. If you are not the > intended recipient, please delete it and notify us immediately. > Please do not copy or use it for any purpose, or disclose its > contents to any other person as it may be an offence under the > Official Secrets Act. Thank you.
