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Hi Phil,
if you have an idea where the missing 20% should be, probably the best
way to get some improved electron density there is to use BUSTER which
allows masking regions of expected electron density in its ML
refinement. I haven't used BUSTER for a while, so please, check
http://www.globalphasing.com for more infos.
Good luck,
Dirk.
Phil Jeffrey wrote:
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Something I have never found to work to my satisfaction has been solvent
flattening after molecular replacement where the model is somewhat
partial (i.e. more than 20% of the new structure is not modeled by the
MR solution). In a case I was trying out for someone else a few months
back I used DM with a variety of automatic and more manually-generated
solvent masks, and this produced no significant gain in the phases to my
eyes. So I am left with the suspicion that I'm not using the best methods.
Does anyone have any advice ? - I've found Coulombe and Cygler's 1997
paper (Acta Cryst D53, 426-433) - but I imagine that there is other
information out there that an hour's Googling has yet to turn up.
Thanks
Phil Jeffrey
Princeton, NJ
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Dirk Kostrewa
Paul Scherrer Institut
Biomolecular Research, OFLC/110
CH-5232 Villigen PSI, Switzerland
Phone: +41-56-310-4722
Fax: +41-56-310-5288
E-mail: [EMAIL PROTECTED]
http://sb.web.psi.ch
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