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Am 25 Aug 2006 um 10:51 hat Phil Jeffrey geschrieben: > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Something I have never found to work to my satisfaction has been > solvent flattening after molecular replacement where the model is > somewhat partial (i.e. more than 20% of the new structure is not > modeled by the MR solution). In a case I was trying out for someone > else a few months back I used DM with a variety of automatic and more > manually-generated solvent masks, and this produced no significant > gain in the phases to my eyes. So I am left with the suspicion that > I'm not using the best methods. > > Does anyone have any advice ? - I've found Coulombe and Cygler's 1997 > paper (Acta Cryst D53, 426-433) - but I imagine that there is other > information out there that an hour's Googling has yet to turn up. > > Thanks > Phil Jeffrey > Princeton, NJ > Dear Phil, I made very good experiences with the solvent flattening and NCS averaging modules of CNS in a case of low resolution (3.1 A; see EMBO J. 20, 5320-5331, 2001). Good luck! Karsten Niefind ----------------------------------------------- Dr. Karsten Niefind Institute of Biochemistry University of Cologne Zuelpicher Strasse 47 D-50674 Cologne tel.: +49/221/470-6444 (Secretary: +49/221/470-6440) fax: +49/221/470-5092 E-mail: [EMAIL PROTECTED] Internet: http://www.hnb-cologne.uni- koeln.de/WWW/m_staff/kni/inhalt_englisch.htm
