*** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk ***
Hi, I'm trying to get a crystal for one protein and so far i can only get some small ones.However the crystals were grown from the perticipate.After I set up the trays, the first several days i only saw perticipitate, and then after 10 days, lots of tiny crystals show up.I tried to use the additive screen, but still didn't change much.In order to get a bigger crystal, what can I do the next step? I was thinking about seeding, is there any protocol for doing this?I'm a little confused about one thing is that after I crushed the crystals, I need to add to the pre-equilibrium solution, does this mean, in my case, I should put the tiny crystals into the trays that I already set up for 10 days?I'm using haning drop right now.Is it easier to use sitting drop instead to do seeding?Any suggestions would be appreciated. Thanks. Jenny
