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I would try microseeding, you can do it on sitting or hanging drops, as you prefer. I prepare some fresh drops, I usually keep the precipitant concentration and I reduce the protein concentration (try several ones: one third, one half...) I leave the drops equilibrate overnight but in some cases a few hours is enough. For me the best probe for seeding is a cat hair, some people use a cat whisker but I think that is too thick and it takes too many seeds. If there is no cat available you can use an accupunture needle but that makes the process less esoteric ;-) What I do it is opening the drop that have the tiny crystals, I don't bother to crash the crystals, I just touch them with the cat hair, that is enough for seeding 5-6 drops. Before dragging the hair through the pre-equilibrated drops, I drag it through a few drops of mother liquour to wash the excess of seeds. That step is very important because if you put too many seeds you will have a shower of tiny crystals. I hope that helps, try different protein concentrations and pre-equilibration times and have faith!! Good luck, Maria
