Hi, Jenny, Design an optimization crystallization screen around your current lead condition. I'd try an eight rows x six columns screen in which the pH varies across the rows and the main precipitant concentration varies down the columns. Set up your protein sample against this screen in a sitting drop plate. Before you seal up the plate, drag a seed probe through one of your drops which contains the small crystals of which you speak. Then take that seed probe and drag it through each of the new drops you set up against the optimization screen. Refresh the seed probe by dragging it through the crystal-containing drop after every six newly set up drops. For the seed probe, the one that Hampton sells is good. If you have enough protein, you should also try this seeding in another round of random screening crystallization trials. Good luck,
Hidong Jenny <[EMAIL PROTECTED]> Sent by: [EMAIL PROTECTED] 11/21/2006 07:24 PM To [email protected] cc Subject [ccp4bb]: seeding? *** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** Hi, I'm trying to get a crystal for one protein and so far i can only get some small ones.However the crystals were grown from the perticipate.After I set up the trays, the first several days i only saw perticipitate, and then after 10 days, lots of tiny crystals show up.I tried to use the additive screen, but still didn't change much.In order to get a bigger crystal, what can I do the next step? I was thinking about seeding, is there any protocol for doing this?I'm a little confused about one thing is that after I crushed the crystals, I need to add to the pre-equilibrium solution, does this mean, in my case, I should put the tiny crystals into the trays that I already set up for 10 days?I'm using haning drop right now.Is it easier to use sitting drop instead to do seeding?Any suggestions would be appreciated. Thanks. Jenny
