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Hi Jenny, Maybe this paper is usefull. Saridakis & Chayen Protein Science (2000), 9:755-757 A simple method for growing protein crystals in the metastable zone using the vapor diffusion technique is described. The coverslips holding the hanging drops are transferred, after being incubated for some time at conditions normally giving many small crystals, over reservoirs at concentrations that normally yield clear drops. Fewer, much larger and better diffracting crystals are obtained, compared with conventional crystallization at similar conditions. To our knowledge, this is the first report of a significant crystal improvement due to "backing off" from nucleation conditions, using the hanging drop method. A correlation of the transfer time with published results for vapor diffusion equilibration of poly(ethylene glycol) solutions is also presented. [ http://www.proteinscience.org/cgi/reprint/9/4/755 ] Cheers Peter ----- Original Message ----- From: Jenny <[EMAIL PROTECTED]> Date: Tuesday, November 21, 2006 7:33 pm Subject: [ccp4bb]: seeding? To: [email protected] > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Hi, > > I'm trying to get a crystal for one protein and so far i can only get > some small ones.However the crystals were grown from the > perticipate.After I set up the trays, the first several days i only > saw perticipitate, and then after 10 days, lots of tiny crystals show > up.I tried to use the additive screen, but still didn't change much.In > order to get a bigger crystal, what can I do the next step? > > I was thinking about seeding, is there any protocol for doing this?I'm > a little confused about one thing is that after I crushed the > crystals, I need to add to the pre-equilibrium solution, does this > mean, in my case, I should put the tiny crystals into the trays > that I > already set up for 10 days?I'm using haning drop right now.Is it > easier to use sitting drop instead to do seeding?Any suggestions would > be appreciated. > > Thanks. > > Jenny >
