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Hi Jenny,
My suggestion is to vary the protein vs well solution volumes. For
example if I am using a 5 ul protein in a drop, I add 5, 4, 3, 2, 1
and 0 ul of precipitant to the drop, while keeping the well solution
constant across.
This method is believed to work in cases like yours where there is a
precipitation first and then crystallization.
Good luck!
Anthony
________________________________
Anthony Addlagatta, PhD
HHMI Research Associate
Institute of Molecular Biology
University of Oregon
Eugene, OR-97403
Phone: (541) 346-5867
Fax: (541)346-5270
Web: http://darkwing.uoregon.edu/~anthony
On Nov 21, 2006, at 7:20 PM, Jenny wrote:
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Hi,
I'm trying to get a crystal for one protein and so far i can only get
some small ones.However the crystals were grown from the
perticipate.After I set up the trays, the first several days i only
saw perticipitate, and then after 10 days, lots of tiny crystals show
up.I tried to use the additive screen, but still didn't change much.In
order to get a bigger crystal, what can I do the next step?
I was thinking about seeding, is there any protocol for doing this?I'm
a little confused about one thing is that after I crushed the
crystals, I need to add to the pre-equilibrium solution, does this
mean, in my case, I should put the tiny crystals into the trays that I
already set up for 10 days?I'm using haning drop right now.Is it
easier to use sitting drop instead to do seeding?Any suggestions would
be appreciated.
Thanks.
Jenny
