I cannot say what you have - running a gel, MS, etc of a washed crystal could confirm what you have. What you did not indicate is if your lack of diffraction was of frozen or unfrozen crystals. I have seen too many cases where it is the cryo condition killing diffraction. So if you have not tried yet - try a wet mount.
Ezra On 8/30/2010 9:24 PM, qiangm zhang wrote: > Hi all, > I got a crystal of one membrane protein (~60kD) from Na/K phosphate > condition (see getit_4), then I got the improved crystal like getit_5 > after trying seeding, different detergents, lipids and additives. But > this crystal does not diffract at all, I already tried Izit staining > which shows it is protein crystal (detergent crystal?). Does anyone > have any good suggestions for the optimization of this membrane > protein crystallization? Thank you in advance. > > Best regards > > Qiangmin Zhang > > Biomedical Science Tower 3 > Room1034 > 3501 Fifth Avenue > Pittsburgh, PA 15260 > > > > > > -- > 张强敏
