I have to say that in my limited experience with membrane protein crystallization, these liquid crystal / spherulite type things are very common, and seldom turn into anything useful. Perhaps others on the list more experienced than I can corroborate or refute this. I just don't want this guy to get misled into perhaps wasting months/years on something not particularly promising. But, as I said, I am happy to be contested/refuted...
Jacob On Tue, Aug 31, 2010 at 11:39 AM, Ed Pozharski <epozh...@umaryland.edu>wrote: > Well said. I've seen three cases by now when switching to a homologue > from a different organism led to solving a structure (and way too many > cases when crystals just did not diffract, either at all or well > enough :). > > On Tue, 2010-08-31 at 18:48 +0300, Tommi Kajander wrote: > > Or might be worth going back to the drawing board to design more > > constructs (and check them around the same conditions), thermostable > > homologs etc.. what about reductive methylation, anyone had luck > > with > > membrane proteins? > > > > -- > "I'd jump in myself, if I weren't so good at whistling." > Julian, King of Lemurs >
