instead of DLS you could also look into buying a fancy PCR machine ~30K where you can perform a) regular PCR b) quantitative PCR c) thermal stability tests for your protein
Ericsson et al. Thermofluor-based high-throughput stability optimization of proteins for structural studies. Anal Biochem (2006) vol. 357 (2) pp. 289-98 Crowther et al. Buffer Optimization of Thermal Melt Assays of Plasmodium Proteins for Detection of Small-Molecule Ligands. Journal of biomolecular screening : the official journal of the Society for Biomolecular Screening (2009) pp. Sure you are missing the quantification for how homogeneous your sample is and an estimation of the molecular weight of the particles in solution but the correlation between crystallizability and DLS is not so good compared to thermal shifts as pointed out in Ericsson 2006. You will have a high end purification system so you could get a sense of your sample homogeneity via SEC instead Just a thought, Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ On Oct 30, 2010, at 12:02 AM, Mohd Shukuri Mohamad Ali wrote: > Hi there > > Sorry for the off ccp4 topics. I am planning to get a DLS to set up my > crystallography lab. I got a tight budget. Around USD 80000 perhaps. I'm > quite new to this field. > > Thanks in advance for your suggestions > > Regards > Shukuri
