hear-hear
wise words.
may I add that if you are buying a DLS anyway, maybe consider getting
a model which can also be connected online to an FPLC to do Static LS
measurements,
... after all a DLS signal is basically an autocorrelation of the
static signal over time ... which will tell you about dispersion,
but is incapable of measuring MW, and can only get Rg, unlike static
LS (MALLS) that will give you absolute MW over your FPLC - which is
handy.
A.
On Oct 30, 2010, at 20:10, Artem Evdokimov wrote:
I second the question of need: a decent PCR machine capable of
'thermofluor'-like experiments should cost around $34-$38K, which
leaves about the same amount of $$ for a decent purification
machine. One of the questions you have to figure out is: are you
setting up a high-throughput lab or a regular kind of lab? If you're
doing HT work then perhaps a plate-based DLS is a good idea,
although you will undoubtedly need more $$ for the rest of the
stuff. On the other hand if you're setting up to do 'regular' i.e.
low-throughput (I prefer the term 'hand crafted', by the way)
purification then an investment in a Thermofluor machine (be it PCR
or a multiwell spectrofluorimeter with temperature control) and
another useful device might be more wise.
Having written this, I would have to add for the sake of honesty
that my lab has both - but we are in fact straddling the boundary
between HT and hand-crafted work and we have use both instruments
daily.
Artem
On Fri, Oct 29, 2010 at 11:02 PM, Mohd Shukuri Mohamad Ali <shuk...@biotech.upm.edu.my
> wrote:
Hi there
Sorry for the off ccp4 topics. I am planning to get a DLS to set up my
crystallography lab. I got a tight budget. Around USD 80000 perhaps.
I'm
quite new to this field.
Thanks in advance for your suggestions
Regards
Shukuri
P please don't print this e-mail unless you really need to
Anastassis (Tassos) Perrakis, Principal Investigator / Staff Member
Department of Biochemistry (B8)
Netherlands Cancer Institute,
Dept. B8, 1066 CX Amsterdam, The Netherlands
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