I second the question of need: a decent PCR machine capable of 'thermofluor'-like experiments should cost around $34-$38K, which leaves about the same amount of $$ for a decent purification machine. One of the questions you have to figure out is: are you setting up a high-throughput lab or a regular kind of lab? If you're doing HT work then perhaps a plate-based DLS is a good idea, although you will undoubtedly need more $$ for the rest of the stuff. On the other hand if you're setting up to do 'regular' i.e. low-throughput (I prefer the term 'hand crafted', by the way) purification then an investment in a Thermofluor machine (be it PCR or a multiwell spectrofluorimeter with temperature control) and another useful device might be more wise.
Having written this, I would have to add for the sake of honesty that my lab has both - but we are in fact straddling the boundary between HT and hand-crafted work and we have use both instruments daily. Artem On Fri, Oct 29, 2010 at 11:02 PM, Mohd Shukuri Mohamad Ali < [email protected]> wrote: > Hi there > > Sorry for the off ccp4 topics. I am planning to get a DLS to set up my > crystallography lab. I got a tight budget. Around USD 80000 perhaps. I'm > quite new to this field. > > Thanks in advance for your suggestions > > Regards > Shukuri >
