Hi Shukuri, If you're on a tight budget and you only want to use DLS to verify sample homogeneity I would save my money or use it for something else (a crystallization robot, for example). You definitely do NOT need DLS to solve crystal structures, including those of membrane proteins. Many monodisperse samples do not crystallize and I can tell you from experience that polydisperse samples can crystallize perfectly well. Gel filtration chromatography is a good and much cheaper alternative to DLS, and you'll have the columns already anyway. Admittedly, DLS will give you somewhat more info than gel filtration, but again, if money is an issue I would not get a DLS instrument. For me its comparable to a UV microscope for verification of crystals; nice for sure, but not worth the $$$.
Cheers, Bert On 10/30/10 12:02 AM, "Mohd Shukuri Mohamad Ali" <[email protected]> wrote: Hi there Sorry for the off ccp4 topics. I am planning to get a DLS to set up my crystallography lab. I got a tight budget. Around USD 80000 perhaps. I'm quite new to this field. Thanks in advance for your suggestions Regards Shukuri
