Dear Len This is a classic sign of osmotic shock. You can try matching the osmotic pressure of the mother liquor and the cryoprotectant buffer. For a protocol see Acta Cryst D (1999) 55, 1649 section 6.4 Good luck Best wishes Elspeth
-----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Leonard Thomas Sent: 26 October 2011 17:46 To: [email protected] Subject: [ccp4bb] cryo protection Hi All, I have run into a very sensitive crystals system when it comes to cryo protecting them. I have run through the usual suspects and trays are going to be setup with a cryo protectant as part of crystallization cocktail. The one problem that seems to be occurring is that the crystals crack as soon as they are transfered out of the original drop. I am running out of ideas and really would love some new ones. Thanks in advance. Len Leonard Thomas Ph.D. Macromolecular Crystallography Laboratory Manager University of Oklahoma Department of Chemistry and Biochemistry Stephenson Life Sciences Research Center 101 Stephenson Parkway Norman, OK 73019-5251 [email protected] http://barlywine.chem.ou.edu Office: (405)325-1126 Lab: (405)325-7571
