I'm going with Jurgen on this one. http://img27.imageshack.us/img27/3232/pastedgraphic1.png
Sad was the day when I mounted this puppy and it shot to 8-10A. Room temperature. And messing around with cryos didn't help either. Can't remember the size, but I think I had scooped it with a 0.8 mm loop. I should've mounted it on a ring and given it to my wife. And that's not chromatic artifact, the ligand was red. On a side note, I had a very small crystal embedded in a chunk of ice at the end of a 0.025 mm loop. Couldn't even see it on the very nice on-axis cameras at the ALS. I shot blindly into the ice.. .it diffracted to about 1.8A (and the ice wasn't bad at all) F On Feb 7, 2012, at 9:52 AM, Bosch, Juergen wrote: > Hi Enrico, > > I was just looking at non-optimal cryo-conditions and the original posters > starting point. > Of course if you have a good cryo bigger is better for the reasons you write > but if you have no clue how your crystals will perform then I'd rather go for > small to be cautious and also have those around and not only the big ones > which everybody mounts because they looks so nice. To be disappointed by big > crystals is often not a surprise to me and if you have not tried small > crystals from the same batch well then you missed 50% of your chances to > solve s structure with the first light the crystals saw. > > Jürgen > > --------------------------------------------- Francis E. Reyes M.Sc. 215 UCB University of Colorado at Boulder
