One last thing--sometimes crystals can be frozen as is, particularly if you use mitegen mounts and get nearly all of the mother liquor off the crystals by dabbing the loop on the dry surface next to the drop several times. So simple it is always worth a try....
JPK On Tue, Feb 7, 2012 at 2:37 AM, Mark J van Raaij <[email protected]> wrote: > Rationalising it completely may only be possible once you know the nature of > the crystal contacts, i.e. when you have solved the structure. Until then it > is mainly a matter of experimenting. >> >> -----Original Message----- >> From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of >> Theresa H. Hsu >> Sent: Monday, February 06, 2012 11:00 PM >> To: [email protected] >> Subject: Re: [ccp4bb] Freezing crystal >> >> Hi all >> >> Thanks for all the suggestions which I will try soon. >> >> How do the crystallization condition (PEG vs. salts like ammonium >> sulfate) affect the croyprotectant condition? Do factors like presence >> of low concentration of high molecular weight PEG (> 2000) mean PEG is >> better? Do buffers and salts in protein also important? >> >> Trying to rationalize it :) >> >> Theresa -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program email: [email protected] *******************************************
