> Sad was the day when I mounted this puppy and it shot to 8-10A. Room > temperature. And messing around with cryos didn't help either.
But if that puppy had been smaller, it might have diffracted even worse, and just think if that little icy crystal had been bigger... JPK > > > Can't remember the size, but I think I had scooped it with a 0.8 mm loop. > > I should've mounted it on a ring and given it to my wife. And that's not > chromatic artifact, the ligand was red. > > On a side note, I had a very small crystal embedded in a chunk of ice at the > end of a 0.025 mm loop. Couldn't even see it on the very nice on-axis > cameras at the ALS. I shot blindly into the ice.. .it diffracted to about > 1.8A (and the ice wasn't bad at all) > > > F > > > On Feb 7, 2012, at 9:52 AM, Bosch, Juergen wrote: > >> Hi Enrico, >> >> I was just looking at non-optimal cryo-conditions and the original posters >> starting point. >> Of course if you have a good cryo bigger is better for the reasons you write >> but if you have no clue how your crystals will perform then I'd rather go >> for small to be cautious and also have those around and not only the big >> ones which everybody mounts because they looks so nice. To be disappointed >> by big crystals is often not a surprise to me and if you have not tried >> small crystals from the same batch well then you missed 50% of your chances >> to solve s structure with the first light the crystals saw. >> >> Jürgen >> >> > > --------------------------------------------- > Francis E. Reyes M.Sc. > 215 UCB > University of Colorado at Boulder -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program email: j-kell...@northwestern.edu *******************************************
