Re: [ccp4bb] Freezing crystal

[Mark J van Raaij]

Rationalising it completely may only be possible once you know the nature of 
the crystal contacts, i.e. when you have solved the structure. Until then it is 
mainly a matter of experimenting.
> 
> -----Original Message-----
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> Theresa H. Hsu
> Sent: Monday, February 06, 2012 11:00 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Freezing crystal
> 
> Hi all
> 
> Thanks for all the suggestions which I will try soon.
> 
> How do the crystallization condition (PEG vs. salts like ammonium
> sulfate) affect the croyprotectant condition? Do factors like presence
> of low concentration of high molecular weight PEG (> 2000) mean PEG is
> better? Do buffers and salts in protein also important?
> 
> Trying to rationalize it :)
> 
> Theresa