Dear All,
One of the most efficient methods to change space group and packing
without having to change
the sequence is to change the length of N and/or C terminal tags.
An example that I am familiar with is given by the following PDB codes.
1JIZ, 1RMZ, 1JK3, 1UTT, 1UTZ, 2WOA, 2W0D, 1ROS, 1OS9, 3BA0
It includes 1 surface residue mutation, but the rest are small variations
in length.
Complexation with any ligand that may protrude is also likely to work.
Enrico.
On Wed, 15 Feb 2012 01:35:36 +0100, Bernhard Rupp (Hofkristallrat a.D.)
<[email protected]> wrote:
http://services.mbi.ucla.edu/SER/
but no space group predictions are possible. BR
From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of
Prem
Kaushal
Sent: Tuesday, February 14, 2012 3:36 PM
To: [email protected]
Subject: [ccp4bb] surface residue mutation
Hi
We have a protein that crystallized in P21212 space group. We are looking
for some different crystal forms. We tried few things did not work. Now
we
are thinking to mutate surface residues. Anybody aware of any software
which
can predict the mutations that might help in crystallizing protein in
different space group, please inform me.
Thanks in advance
Prem
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: [email protected] Fax: 33 (0)1 69 08 90 71
