Dear all, Thanks for your response to my quarry about surface residue mutation. I tried the SERp Server, but did not get satisfactory clue. This software classified our protein as difficult to crystallize, however we have crystallized and solved the structure also. But our crystal form is not good for soaking experiments. Wondering is there any other software.
Thanks Prem On Thu, Feb 16, 2012 at 3:56 PM, Joel Tyndall <[email protected]>wrote: > Steve Kent has published a few more (at least 1 other) since HIV... > > 3ODV http://www.rcsb.org/pdb/explore/explore.do?structureId=3ODV > > Total chemical synthesis and X-ray structure of kaliotoxin by racemic > protein crystallography. > > Pentelute, B.L., Mandal, K., Gates, Z.P., Sawaya, M.R., Yeates, T.O., > Kent, S.B., > > Journal: (2010) Chem.Commun.(Camb.) 46: 8174-8176 > > _________________________________ > Joel Tyndall, PhD > > Senior Lecturer in Medicinal Chemistry > National School of Pharmacy > University of Otago > PO Box 56 Dunedin 9054 > New Zealand > Skype: jtyndall > http://www.researcherid.com/rid/C-2803-2008 > Pukeka Matua > Te Kura Taiwhanga Putaiao > Te Whare Wananga o Otago > Pouaka Poutapeta 56 Otepoti 9054 > Aotearoa > > Ph / Waea +64 3 4797293 > Fax / Waeawhakaahua +64 3 4797034 > > > -----Original Message----- > From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of > Jacob Keller > Sent: Thursday, 16 February 2012 7:36 a.m. > To: [email protected] > Subject: Re: [ccp4bb] surface residue mutation > > Right on the money! > > JPK > > On Wed, Feb 15, 2012 at 12:28 PM, David Schuller <[email protected]> > wrote: > > On 02/15/12 12:41, Jacob Keller wrote: > >>> > >>> Are there any all-D proteins out there, of known structure or > >>> otherwise? If so, do enantiomer-specific catalyses become inverted? > >>> > >>> JPK > >>> > > I looked a little harder, and at least one D-enantiomeric protein was > > an > > enzyme: > > > > Total chemical synthesis of a D-enzyme: the enatiomers of HIV-1 > > protease show demonstration of reciprocal chiral substrate specificty > > R.C. deL. Milton, S.C.F. Milton, S.B.H. Kent (1992) Science 256(5062) > > 1445-1448. > > > > I guess that answers your question. > > > > > > -- > > ====================================================================== > > = > > All Things Serve the Beam > > ====================================================================== > > = > > David J. Schuller > > modern man in a post-modern world > > MacCHESS, Cornell University > > [email protected] > > > > -- > ******************************************* > Jacob Pearson Keller > Northwestern University > Medical Scientist Training Program > email: [email protected] > ******************************************* > -- Prem S. Kaushal Post Doctoral Fellow School of Medicine Department of Pharmacology Case Western Reserve University Cleveland OHIO 44106 2164328447
