regaentDear All,
I am sure this question was discussed before. But I am wondering if anyone got
the same experience as I do. I got a crystal out of condition with 1M KCl, 1.4M
Ammonium sulfate at pH7. I tried to use glycerol, ethylene glycol, 25% sucrose,
paraton-N oil, or ammonium sulfate itself: The problem is that all the cryo
plus original reagents in the reservoir precipitate the salts out. And more
serious problem is because of high salt in the condition, while I am trying to
loop the crystal, both the drop and cryoprotectant drop form salt crystals (not
sure it is KCl or ammonia sulfate) significantly and very quickly, that cause
my crystal dissolved. My crystal doesn't seem to survive paraton-N oil. Does
anyone here have similiar case? any suggestion will be appreciated.
Thanks,Min
