Pressure cryocooling lowers the need for cryoprotectant dramatically.
On 07/10/12 12:28, m zhang wrote:
regaentDear All,
I am sure this question was discussed before. But I am wondering if
anyone got the same experience as I do.
I got a crystal out of condition with 1M KCl, 1.4M Ammonium sulfate at
pH7. I tried to use glycerol, ethylene glycol, 25% sucrose, paraton-N
oil, or ammonium sulfate itself: The problem is that all the cryo plus
original reagents in the reservoir precipitate the salts out. And more
serious problem is because of high salt in the condition, while I am
trying to loop the crystal, both the drop and cryoprotectant drop form
salt crystals (not sure it is KCl or ammonia sulfate) significantly
and very quickly, that cause my crystal dissolved. My crystal doesn't
seem to survive paraton-N oil. Does anyone here have similiar case?
any suggestion will be appreciated.
Thanks,
Min
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All Things Serve the Beam
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David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
schul...@cornell.edu
