Hi there,
You could try to go for liquid-liquid interface crystallisation (in
capillaries). The minimum volume would be no less than 1.5 microl
protein + 1.5 microl precipitant, i.e. not the volumes used by nanodrop
robots. Crystallisation under oil (or similar liquids) should also have
the same effect (no spreading out).
HTH,
Fred.
On 07/11/12 13:07, Eva Bligt-Lindén wrote:
Dear ccp4 users,
I have a problem in the crystallization of my target protein. Whenever
I set up a vapour diffusion experiment, either hanging or sitting
drops, the drops spread out. The surface tension is completely lost in
80-90% of the droplets. Have any one experienced something similar?
What could be the reason for this strange behaviour? I have tried
three different commercial screens with 96 condition each and there is
no difference between the screens. There is no difference between
manual or robotic setups either. The protein buffer is 40 mM Tris, 2
mM MgCl2 buffer, pH 7.4. The buffer controls are all ok.
Kind regards,
Eva
____________________________________
Eva Bligt-Lindén (M.Sc.)
PhD student
Structural Bioinformatics Laboratory
Department of Biosciences,
Åbo Akademi University
BioCity, Tykistökatu 6A
FI-20520 Turku
Finland
--
Fred. Vellieux (B.Sc., Ph.D., hdr)
IBS / ELMA
41 rue Jules Horowitz
F-38027 Grenoble Cedex 01
Tel: +33 438789605
Fax: +33 438785494
