Those are the EasyXtal 15 well tools with the EasyXtal DG-Crystal Supports. Look at this link to find them: http://www.qiagen.com/products/protein/crystallization/default.aspx
I agree that they keep the drops from spreading out, but I have experienced trouble harvesting smaller crystals from these xtal tools. Good luck! Bryan From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Rob Gillespie Sent: Wednesday, November 07, 2012 9:19 AM To: [email protected] Subject: Re: [ccp4bb] Problems in crystallization Qiagen used to sell hanging-drop trays with screw-cap tops, and the tops had six rings on them (3 large, 3 small). The rings had a lip on them that kept the drop from spreading beyond the edge. I found these quite useful when setting up drops that had detergent in them. I unfortunately don't know what they are called, as I have several at my bench but not the packaging they arrived in. On Wed, Nov 7, 2012 at 9:04 AM, Eva Bligt-Lindén <[email protected]> wrote: Thank you for your replies. To my knowledge the protein is not a surface-tension-reducing-protein and there is no detergent in the sample. I have tried different plates and still the same result. I will try a different crystallization technique such as batch under oil or counter-diffusion if I do not solve this problem. But now I am curious to know what is causing the disturbed surface tension and if someone else has experienced and dealt with something similar. Kind regards, Eva Quoting anna anna <[email protected]>: Why don't you try batch under oil? 2012/11/7 Eva Bligt-Lindén <[email protected]> Dear ccp4 users, I have a problem in the crystallization of my target protein. Whenever I set up a vapour diffusion experiment, either hanging or sitting drops, the drops spread out. The surface tension is completely lost in 80-90% of the droplets. Have any one experienced something similar? What could be the reason for this strange behaviour? I have tried three different commercial screens with 96 condition each and there is no difference between the screens. There is no difference between manual or robotic setups either. The protein buffer is 40 mM Tris, 2 mM MgCl2 buffer, pH 7.4. The buffer controls are all ok. Kind regards, Eva ______________________________**______ Eva Bligt-Lindén (M.Sc.) PhD student Structural Bioinformatics Laboratory Department of Biosciences, Åbo Akademi University BioCity, Tykistökatu 6A FI-20520 Turku Finland ____________________________________ Eva Bligt-Lindén (M.Sc.) PhD student Structural Bioinformatics Laboratory Department of Biosciences, Åbo Akademi University BioCity, Tykistökatu 6A FI-20520 Turku Finland -------------------------------------------------------------------------- Confidentiality Notice: This message is private and may contain confidential and proprietary information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorized use or disclosure of the contents of this message is not permitted and may be unlawful.
