Why don't you try batch under oil? 2012/11/7 Eva Bligt-Lindén <eva.bl...@abo.fi>
> Dear ccp4 users, > > I have a problem in the crystallization of my target protein. Whenever I > set up a vapour diffusion experiment, either hanging or sitting drops, the > drops spread out. The surface tension is completely lost in 80-90% of the > droplets. Have any one experienced something similar? What could be the > reason for this strange behaviour? I have tried three different commercial > screens with 96 condition each and there is no difference between the > screens. There is no difference between manual or robotic setups either. > The protein buffer is 40 mM Tris, 2 mM MgCl2 buffer, pH 7.4. The buffer > controls are all ok. > > Kind regards, > Eva > > ______________________________**______ > > Eva Bligt-Lindén (M.Sc.) > PhD student > Structural Bioinformatics Laboratory > > Department of Biosciences, > Åbo Akademi University > BioCity, Tykistökatu 6A > FI-20520 Turku > Finland >
