I would recommend the Microcal ITC 200, hands down. Not only is it an amazing instrument with the optional automated sample loader (which is worth every penny), but we were able to do experiments (multiple) using FULL-LENGTH p53 binding to a weak cognate protein. I believe this was the first time ITC was ever used with full length p53, as it is so labile and just loves immediately to oligomerize. Sample sizes pay for the instrument. Best, John
John Fisher, M.D./PhD St. Jude Children's Research Hospital Department of Oncology Department of Structural Biology W: 901-595-6193 C: 901-409-5699 On Mar 23, 2013, at 4:45 AM, Sameh Soror <[email protected]> wrote: > Dear All, > > > I am sorry for the off topic question. I am going to buy ITC to study > protein-protein & protein-ligand interactions.... > > I am comparing microcal, GE and nanoITC, TA instrument.. > any suggestions, recommendations, good experiences or bad experiences. is > there a better system. > > > Thank in advance for the help. > > > Regards > > > Sameh > > -- > Sameh Soror > > Postdoc. fellow > >
