George, would you please explain your comments? We've found the TA Instruments analysis software very robust and user friendly.
We have the low volume nanoITC from TA instruments and get equivalent #'s in our comparison tests to the Microcal instrument. Cheers, Chris -- Christopher L. Colbert, Ph.D. Assistant Professor Department of Chemistry and Biochemistry North Dakota State University P.O. Box 6050 Dept. 2710 Fargo, ND 58108-6050 PH: (701) 231-7946 FAX: (701) 231-8324 On 3/23/13 8:47 AM, "George Kontopidis" <[email protected]> wrote: >Keep in mind that output files from nanoITC, TA instrument cannot be red >by >Origin. At some point you will need to analyse your data further. > >George > >-----Original Message----- >From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of >Anastassis Perrakis >Sent: Saturday, March 23, 2013 12:46 PM >To: [email protected] >Subject: Re: [ccp4bb] Isothermal titration calorimetry > >It might be worth to consider the question more in detail. > >Do you want to study thermodynamics of the interaction, or a KD would do? >If >the former, you need ITC. If the latter, and you want to study things at >the >level of KD only, maybe investing on a plate reader, thermophoresis, or >some >biosensor technology (spr or interferometry based systems) should be >considered. > >Then, what interactions will you study with the ITC? In general, I would >agree that the lower sample volume is worth the nano options, but >depending >on the typical systems under study, sometimes the gain on sample quantity >is >not worth the money - while many times its worth it. > >John is if course right that for studying specific systems as the one he >describes the 200 is great. > >A. > >Sent from my iPhone > >On 23 Mar 2013, at 11:00, John Fisher <[email protected]> wrote: > >> I would recommend the Microcal ITC 200, hands down. Not only is it an >amazing instrument with the optional automated sample loader (which is >worth >every penny), but we were able to do experiments (multiple) using >FULL-LENGTH p53 binding to a weak cognate protein. I believe this was the >first time ITC was ever used with full length p53, as it is so labile and >just loves immediately to oligomerize. Sample sizes pay for the >instrument. >> Best, >> John >> >> John Fisher, M.D./PhD >> St. Jude Children's Research Hospital >> Department of Oncology >> Department of Structural Biology >> W: 901-595-6193 >> C: 901-409-5699 >> >> On Mar 23, 2013, at 4:45 AM, Sameh Soror <[email protected]> >>wrote: >> >>> Dear All, >>> >>> >>> I am sorry for the off topic question. I am going to buy ITC to study >protein-protein & protein-ligand interactions.... >>> >>> I am comparing microcal, GE and nanoITC, TA instrument.. >>> any suggestions, recommendations, good experiences or bad experiences. >>>is >there a better system. >>> >>> >>> Thank in advance for the help. >>> >>> >>> Regards >>> >>> >>> Sameh >>> >>> -- >>> Sameh Soror >>> >>> Postdoc. fellow >>> >>> >
