This might have changed, but in the past file formats were different.   
Microcal files are text, while TA's are binary.  I do have the actual 
description of TA's format if anyone is interested, but it must be easier to 
use native text export than write a converter. 

-------- Original message --------
From: "Bosch, Juergen" <jubo...@jhsph.edu> 
Date:  
To: CCP4BB@JISCMAIL.AC.UK 
Subject: Re: [ccp4bb] Isothermal titration calorimetry 
 
Hi George,
this is probably a very stupid suggestion and you likely have tried it, but 
I'll suggest the obvious nevertheless.
What happens to your .nitc file when you rename it to .itc can you read it in 
Origin then ?

Jürgen


On Mar 24, 2013, at 6:39 AM, George Kontopidis wrote:

Chris,  indeed nanoITC  instrument analysis software is very robust and user
friendly (probable more friendly than microcal, GE). 

Although when you need  to subtract  Q (heat)  values (from 2 or 3 blank
experiments) from your experimental data you cannot. NanoITC software  can
subtract  Q values only  from 1 blank experiment.
Also if you want to present  your data in a form of  heat/mol in Y
(vertical) axes  again you cannot. It presents  data in Y axes only in form
of heat/injection. 
If you have found a way to extract 2 or 3 blank experiments from
experimental data or present data in form of heat/mol, please let me know it
will be very useful.

The main problem in the output files from nanoITC come with an extension
.nitc, by default.  Unfortunately Origin (that can do all the above) can
read only,  filenames with an extension .itc

Cheers,

George

-----Original Message-----
From: Colbert, Christopher [mailto:christopher.colb...@ndsu.edu] 
Sent: Saturday, March 23, 2013 5:56 PM
To: George Kontopidis; CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Isothermal titration calorimetry


George, would you please explain your comments?  We've found the TA
Instruments analysis software very robust and user friendly.

We have the low volume nanoITC from TA instruments and get equivalent #'s in
our comparison tests to the Microcal instrument.

Cheers,

Chris


--
Christopher L. Colbert, Ph.D.
Assistant Professor
Department of Chemistry and Biochemistry North Dakota State University P.O.
Box 6050 Dept. 2710 Fargo, ND 58108-6050
PH: (701) 231-7946
FAX: (701) 231-8324





On 3/23/13 8:47 AM, "George Kontopidis" <gkontopi...@vet.uth.gr> wrote:

Keep in mind that output files from  nanoITC, TA instrument cannot be 
red by Origin. At some point you will need to analyse your data 
further.

George

-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of 
Anastassis Perrakis
Sent: Saturday, March 23, 2013 12:46 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Isothermal titration calorimetry

It might be worth to consider the question more in detail.

Do you want to study thermodynamics of the interaction, or a KD would do?
If
the former, you need ITC. If the latter, and you want to study things
at the level of KD only, maybe investing on a plate reader, 
thermophoresis, or some biosensor technology (spr or interferometry 
based systems) should be considered.

Then, what interactions will you study with the ITC? In general, I 
would agree that the lower sample volume is worth the nano options, but 
depending on the typical systems under study, sometimes the gain on 
sample quantity is not worth the money - while many times its worth it.

John is if course right that for studying specific systems as the one 
he describes the 200 is great.

A. 

Sent from my iPhone

On 23 Mar 2013, at 11:00, John Fisher <johncfishe...@gmail.com> wrote:

I would recommend the Microcal ITC 200, hands down. Not only is it an
amazing instrument with the optional automated sample loader (which is 
worth every penny), but we were able to do experiments (multiple) using 
FULL-LENGTH p53 binding to a weak cognate protein. I believe this was 
the first time ITC was ever used with full length p53, as it is so 
labile and just loves immediately to oligomerize. Sample sizes pay for 
the instrument.
Best,
John

John Fisher, M.D./PhD
St. Jude Children's Research Hospital Department of Oncology
Department of Structural Biology
W: 901-595-6193
C: 901-409-5699

On Mar 23, 2013, at 4:45 AM, Sameh Soror <shamd...@googlemail.com>
wrote:

Dear All,


I am sorry for the off topic question. I am going to buy ITC to
study
protein-protein & protein-ligand interactions....

I am comparing microcal, GE and nanoITC, TA instrument..
any suggestions, recommendations, good experiences or bad experiences.
is
there a better system.


Thank in advance for the help.


Regards


Sameh

--
Sameh Soror

Postdoc. fellow




......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab:      +1-410-614-4894
Fax:      +1-410-955-2926
http://lupo.jhsph.edu




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