Keep in mind that output files from nanoITC, TA instrument cannot be red by Origin. At some point you will need to analyse your data further.
George -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Anastassis Perrakis Sent: Saturday, March 23, 2013 12:46 PM To: [email protected] Subject: Re: [ccp4bb] Isothermal titration calorimetry It might be worth to consider the question more in detail. Do you want to study thermodynamics of the interaction, or a KD would do? If the former, you need ITC. If the latter, and you want to study things at the level of KD only, maybe investing on a plate reader, thermophoresis, or some biosensor technology (spr or interferometry based systems) should be considered. Then, what interactions will you study with the ITC? In general, I would agree that the lower sample volume is worth the nano options, but depending on the typical systems under study, sometimes the gain on sample quantity is not worth the money - while many times its worth it. John is if course right that for studying specific systems as the one he describes the 200 is great. A. Sent from my iPhone On 23 Mar 2013, at 11:00, John Fisher <[email protected]> wrote: > I would recommend the Microcal ITC 200, hands down. Not only is it an amazing instrument with the optional automated sample loader (which is worth every penny), but we were able to do experiments (multiple) using FULL-LENGTH p53 binding to a weak cognate protein. I believe this was the first time ITC was ever used with full length p53, as it is so labile and just loves immediately to oligomerize. Sample sizes pay for the instrument. > Best, > John > > John Fisher, M.D./PhD > St. Jude Children's Research Hospital > Department of Oncology > Department of Structural Biology > W: 901-595-6193 > C: 901-409-5699 > > On Mar 23, 2013, at 4:45 AM, Sameh Soror <[email protected]> wrote: > >> Dear All, >> >> >> I am sorry for the off topic question. I am going to buy ITC to study protein-protein & protein-ligand interactions.... >> >> I am comparing microcal, GE and nanoITC, TA instrument.. >> any suggestions, recommendations, good experiences or bad experiences. is there a better system. >> >> >> Thank in advance for the help. >> >> >> Regards >> >> >> Sameh >> >> -- >> Sameh Soror >> >> Postdoc. fellow >> >>
