Dear All, i have solved a structure of 3.2 A. That is a PLP depended enzyme. In their resting state, PLP-dependent enzymes are usually joined by a covalent aldimine linkage to an essential lysine residue with a C=N bond. This generates the so-called internal aldimine moiety. Could anybody tell me how we can say that this PLP is covalently attached to the Lys or its making Schiff base or not.
-- WITH REGARDS Rohit Kumar Singh Lab. no. 430, P.I. Dr. S. Gourinath, School of Life Sciences, Jawaharlal Nehru University New Delhi -110067
