Hi Prashant, You need to go really slow when concentrating, not more than 2000 rpm for proteins that precipitate easily. Your protein could be sticking to the sides of your centricon or precipitating. Follow the manual in getting your protein out of the filter (I believe you invert the filter into a clean centricon and spin down fast).
If you see the protein precipitating (you see the white chunks), you’ve been going too fast. Spin your protein down in a conical tube to get the precipitation at the bottom, decant supernatant and check the concentration of this supernatant to see if further concentration on this sample is worthwhile. If not, start with a fresh sample. Good luck, Remie On Aug 19, 2014, at 1:42 PM, Prashant Deshmukh <prashantbiophys...@gmail.com> wrote: > Hi, > i am concentrating my protein using centricon filter, but it is precipitated > soon. Please help me solving this problem. > Thanks. > Prashant Deshmukh > Dept. of Biophysics, > NIMHANS, > Bangalore 560 029, > E-mail:prashantbiophys...@gmail.com > Mob.No.: +919620986525
