Some things to try to increase solubility: 1. Move the buffer pH away from the expected pI. Proteins have minimum solubility near their pI values. 2. Add solubilizing agents to the solution, e.g., 20-50% glycerol. (This may alter you crystallization screening strategy) 3. Include some inert salt in the solution to minimize electrostatic interactions, e.g. 100-500 mM NaCl.
Ultimately, your protein just may not be very soluble. That is potentially OK...it will ppt and maybe xtallize well at low concentration. Roger Rowlett On Aug 19, 2014 1:52 PM, "Prashant Deshmukh" <[email protected]> wrote: > Hi, > i am concentrating my protein using centricon filter, but it is > precipitated soon. Please help me solving this problem. > Thanks. > Prashant Deshmukh > Dept. of Biophysics, > NIMHANS, > Bangalore 560 029, > E-mail:[email protected] > Mob.No.: +919620986525 >
