Hi all,
I have a structure at 3.0 A and R/Rfree of 24/28. The mean B value is
around 98. The B value is especially high at the N terminus and two loop
regions (around 120-150 AA).
The rest of the structure averaged around 70-75. Has anyone faced such a
scenario? How reliable is the structure and is there any way to better the
B factors.
Any help is appreciated.
Thank you!!

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