I'm sorry but I don't understand what your problem is. Do you think the B factors are too small for a 3A data set? A range of 70 to 75 is a little smaller than usual but probably not out of bounds.
Dale Tronrud On 10/12/2016 7:59 PM, sunanda williams wrote: > Hi all, > I have a structure at 3.0 A and R/Rfree of 24/28. The mean B value is > around 98. The B value is especially high at the N terminus and two loop > regions (around 120-150 AA). > The rest of the structure averaged around 70-75. Has anyone faced such a > scenario? How reliable is the structure and is there any way to better > the B factors. > Any help is appreciated. > Thank you!!