Sorry for not making the problem clear!
The overall B factor for the 3.0 A structure is around 98.00 A*2. Most of
the deposited structures in the PDB site around this resolution
has an av B around 70 A*2....
All other statistics looks fine. I got a warning message while trying to
upload the structure in PDB that 98 was higher than the norm!
The reason why the structure has such a high B factor could be due to
disordered loops... All the same I was wondering how acceptable this
structure would be to picky reviewers..
And 'better the B factors' means bring them down...sorry couldn't phrase it
'better'! I am going to try doing refinement with group B factors! Would
that help?
Thanks Robbie, I think this I need to make the best of the model I have.
Prof. Sekar will try PDB_redo..Thanks!

On Thu, Oct 13, 2016 at 11:41 AM, Pavel Afonine <> wrote:

> I fully agree with Dale in not understanding what the problem is. Perhaps
> I have a better chance if you clearly explain what exactly you mean by "is
> there any way to better the B factors".
> Pavel
> On Thu, Oct 13, 2016 at 12:57 PM, Dale Tronrud <>
> wrote:
>>    I'm sorry but I don't understand what your problem is.  Do you think
>> the B factors are too small for a 3A data set?  A range of 70 to 75 is a
>> little smaller than usual but probably not out of bounds.
>> Dale Tronrud
>> On 10/12/2016 7:59 PM, sunanda williams wrote:
>> > Hi all,
>> > I have a structure at 3.0 A and R/Rfree of 24/28. The mean B value is
>> > around 98. The B value is especially high at the N terminus and two loop
>> > regions (around 120-150 AA).
>> > The rest of the structure averaged around 70-75. Has anyone faced such a
>> > scenario? How reliable is the structure and is there any way to better
>> > the B factors.
>> > Any help is appreciated.
>> > Thank you!!

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