Hi Rajnandani,
The cheaper DIY protocol that Herman referred to, is actually still in use by us. Use a siliconizing solution (e.g. Serva 35130.02 silicone solution in isopropanol). Put about 20-30 microliters on the coverslips, preferrably with a repeating pipette and in a fumehood. Then put the coverslips in an oven at 110*C for about 75 minutes. Best, Tjaard Dr. Ing. Tjaard Pijning Structural Biology Group Faculty of Science and Engineering, University of Groningen 9747 AG Groningen, The Netherlands +31(0)50 363 4385 | [email protected] From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of [email protected] Sent: 31 januari 2019 10:17 To: [email protected] Subject: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization? A long time ago, before siliconized coverslips became commercially available, we used to siliconize coverslips ourselves. It is not really that much work and unsiliconized cover slips should be very cheap. If you wish, I could try to find back the protocol. Best, Herman Von: CCP4 bulletin board [mailto:[email protected]] Im Auftrag von Rajnandani Kashyap Gesendet: Donnerstag, 31. Januar 2019 09:17 An: [email protected] Betreff: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization? Dear All I am a PhD student who requires lots of coverslips (!!) for setting up hanging drop crystallization. The company sells it for a huge amount. Also there is a wide monetary difference between a normal siliconized coverslip and a 22mm siliconized circle coverslips. We tried to search for an alternative companies but couldn't get any one who sells coverslips with the same dimensions (0.19-0.22mm glass thickness and 22 mm glass diameter). Is there any alternative company (distribution in India) from where we can buy them for a reasonable price? Thanks in advance for sparing your valuable time and efforts. Regards Rajnandani Kashyap India _____ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=YlILrL-rg1OZBp9DSEAcOqrUs8BY0vFG_gPlaAB3qNk&s=t6ZqJh8ledXY32ENHFaeBd9vgbGKNUke1ROmyJzdM6k&e=> &A=1 _____ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> &A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
