Yes, that was it! I just couldn’t remember the name. > On 31 Jan 2019, at 17:04, Mathews, Irimpan I. > <00002add487f8799-dmarc-requ...@jiscmail.ac.uk> wrote: > > Long back we used Prosil solution (I think, 1% solution) for siliconization. > Mathews > > > -----Original Message----- > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of > Goldman, Adrian > Sent: Thursday, January 31, 2019 8:43 AM > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Is there any alternative to siliconized glass > coverslips for crystallization? > > When I was a graduate student, about 150,000 years ago, we took regular > coverslips and doused them in ?silane to make siliconised ones. You then let > them sit in a rack to dry. It was a bit tedious but not horrendously so. > After a while, I stopped doing it altogether, because IMHO it didn’t make a > massive amount of difference to the behaviour of the solution on the cover > slip. It would bead up, or not, depending on what it was. > > So my advice would be: 1) siliconise yourself; 2) compare siliconised versus > non and decide if you can be bothered. > > Adrian Goldman > > > > On 31 Jan 2019, at 16:02, Holton, James M > <0000270165b9f4cf-dmarc-requ...@jiscmail.ac.uk> wrote: > > plastic. > > Plastic cover slips are no good for UV or polarization, but they are > way better than glass if you happen to want to try in-situ diffraction. > (https://doi.org/10.1107/S0021889800001254) > > If you can't afford commercial ones, then you can always cut up some > inkjet transparency film sheets like McPherson did in the above reference. > Then after you've made a few hundred you can ask yourself how much you'd be > willing to pay somebody else to do it for you. There is no wrong answer to > that question, but it will determine which route you take. > > -James Holton > MAD Scientist > > > On 1/31/2019 12:17 AM, Rajnandani Kashyap wrote: > > > Dear All > I am a PhD student who requires lots of coverslips (!!) for > setting up hanging drop crystallization. The company sells it for a huge > amount. Also there is a wide monetary difference between a normal siliconized > coverslip and a 22mm siliconized circle coverslips. We tried to search for an > alternative companies but couldn't get any one who sells coverslips with the > same dimensions (0.19-0.22mm glass thickness and 22 mm glass diameter). Is > there any alternative company (distribution in India) from where we can buy > them for a reasonable price? > Thanks in advance for sparing your valuable time and efforts. > > Regards > Rajnandani Kashyap > > India > > > ________________________________ > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > > ________________________________ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > > ________________________________ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
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