Long back we used Prosil solution (I think, 1% solution) for siliconization. Mathews
-----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Goldman, Adrian Sent: Thursday, January 31, 2019 8:43 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization? When I was a graduate student, about 150,000 years ago, we took regular coverslips and doused them in ?silane to make siliconised ones. You then let them sit in a rack to dry. It was a bit tedious but not horrendously so. After a while, I stopped doing it altogether, because IMHO it didn’t make a massive amount of difference to the behaviour of the solution on the cover slip. It would bead up, or not, depending on what it was. So my advice would be: 1) siliconise yourself; 2) compare siliconised versus non and decide if you can be bothered. Adrian Goldman On 31 Jan 2019, at 16:02, Holton, James M <0000270165b9f4cf-dmarc-requ...@jiscmail.ac.uk> wrote: plastic. Plastic cover slips are no good for UV or polarization, but they are way better than glass if you happen to want to try in-situ diffraction. (https://doi.org/10.1107/S0021889800001254) If you can't afford commercial ones, then you can always cut up some inkjet transparency film sheets like McPherson did in the above reference. Then after you've made a few hundred you can ask yourself how much you'd be willing to pay somebody else to do it for you. There is no wrong answer to that question, but it will determine which route you take. -James Holton MAD Scientist On 1/31/2019 12:17 AM, Rajnandani Kashyap wrote: Dear All I am a PhD student who requires lots of coverslips (!!) for setting up hanging drop crystallization. The company sells it for a huge amount. Also there is a wide monetary difference between a normal siliconized coverslip and a 22mm siliconized circle coverslips. We tried to search for an alternative companies but couldn't get any one who sells coverslips with the same dimensions (0.19-0.22mm glass thickness and 22 mm glass diameter). Is there any alternative company (distribution in India) from where we can buy them for a reasonable price? Thanks in advance for sparing your valuable time and efforts. Regards Rajnandani Kashyap India ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
