I’m sure James H. Is preparing a philosophical dissertation on the "State of the atoms to B or not to B that is not only a refinement question” that he will share momentarily with the board.
Jürgen > On Mar 10, 2023, at 12:06 PM, DEBANU DAS <[email protected]> > wrote: > > Yes, zero occupancy would reflect that. But not the coordinates in any proper > way. > Debanu > > On Fri, Mar 10, 2023 at 8:58 AM Jurgen Bosch <[email protected] > <mailto:[email protected]>> wrote: >> Going back to RIP phasing methods :-) >> So Harry in your particular case occupancy of zero would actually reflect >> reality for those “combusted” atoms. >> >> Jürgen >> >> > On Mar 10, 2023, at 11:56 AM, Harry Powell >> > <[email protected] >> > <mailto:[email protected]>> wrote: >> > >> > Hi folks >> > >> > One other thing that I haven’t noticed anyone mentioning yet (sorry to >> > those who have mentioned it!!) is that you may not see your sidechain >> > atoms in density because they are not there at all, in spite of what you >> > may have had in the original protein, or even if the atoms were really >> > there in the crystal _before_ exposure to the beam. >> > >> > The coordinates are supposed to be what you actually find, not what you >> > hope is there. >> > >> > Just my two ha’porth >> > >> > Harry >> > >> > ######################################################################## >> > >> > To unsubscribe from the CCP4BB list, click the following link: >> > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > >> > This message was issued to members of www.jiscmail.ac.uk/CCP4BB >> > <http://www.jiscmail.ac.uk/CCP4BB>, a mailing list hosted by >> > www.jiscmail.ac.uk <http://www.jiscmail.ac.uk/>, terms & conditions are >> > available at https://www.jiscmail.ac.uk/policyandsecurity/ >> >> ######################################################################## >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> >> This message was issued to members of www.jiscmail.ac.uk/CCP4BB >> <http://www.jiscmail.ac.uk/CCP4BB>, a mailing list hosted by >> www.jiscmail.ac.uk <http://www.jiscmail.ac.uk/>, terms & conditions are >> available at https://www.jiscmail.ac.uk/policyandsecurity/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
