Hello everyone, I am looking for advice on my fragment soaking protocol. Recently, I obtained a crystal structure of a small molecule within my protein. These compounds are insoluble in aqueous solution so I've been preparing aliquots of the compound at known concentrations in 100% DMSO. I aliquot the compounds onto pedestal of sitting drop trays then wait for the DMSO to evaporate before adding my cryo-protectant solution and pre-grown protein crystals. I am trying to reproduce my results with different concentrations of compound but have to wait a long time for the DMSO to evaporate. I am looking for advice on how to speed up the DMSO evaporation.
Thanks, Leon ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
