Cleaver idea. Can you dissolve it in something else? Methanol, for example? DMSO is very hard to get rid of by evaporation.
> On May 12, 2026, at 12:16 PM, Giang, Leon (MU-Student) > <[email protected]> wrote: > > Hello everyone, > > I am looking for advice on my fragment soaking protocol. Recently, I obtained > a crystal structure of a small molecule within my protein. These compounds > are insoluble in aqueous solution so I've been preparing aliquots of the > compound at known concentrations in 100% DMSO. I aliquot the compounds onto > pedestal of sitting drop trays then wait for the DMSO to evaporate before > adding my cryo-protectant solution and pre-grown protein crystals. I am > trying to reproduce my results with different concentrations of compound but > have to wait a long time for the DMSO to evaporate. I am looking for advice > on how to speed up the DMSO evaporation. > > Thanks, > Leon > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 --------------------------------------------------------------------------------------- Patrick J. Loll, Ph. D. (he, him, his) Professor of Biochemistry & Molecular Biology Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St., Mailstop 497 Philadelphia, PA 19102 USA (215) 762-7706 [email protected] [email protected] ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
