Have you tried vacuum? We did solvent mapping years ago and found that DMSO was a good solvent/fragment that resolved well in crystal structures.
Cheers, Quyen Quyen Hoang, PhD Professor of Biochemistry, Molecular Biology, and Pharmacology Director of IUSM Center for Electron Microscopy (iCEM) Adjunct Professor of Neurology Primary Investigator of the Stark Neuroscience Research Institute Indiana University School of Medicine 635 Barnhill Drive, MS4017A Indianapolis, IN, 46202 (317)274-4371 https://qqhoang.pages.iu.edu > On May 12, 2026, at 12:16 PM, Giang, Leon (MU-Student) > <[email protected]> wrote: > > Hello everyone, > > I am looking for advice on my fragment soaking protocol. Recently, I obtained > a crystal structure of a small molecule within my protein. These compounds > are insoluble in aqueous solution so I've been preparing aliquots of the > compound at known concentrations in 100% DMSO. I aliquot the compounds onto > pedestal of sitting drop trays then wait for the DMSO to evaporate before > adding my cryo-protectant solution and pre-grown protein crystals. I am > trying to reproduce my results with different concentrations of compound but > have to wait a long time for the DMSO to evaporate. I am looking for advice > on how to speed up the DMSO evaporation. > > Thanks, > Leon > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
