Indeed, XChem people have found that it’s rarely NOT possible to make the crystal tolerate at least some DMSO – it just “just” requires systematic testing.
From: CCP4 bulletin board <[email protected]> On Behalf Of Jon Cooper Sent: 22 May 2026 14:17 To: [email protected] Subject: Re: [ccp4bb] Fragment soaking protein crystals with small molecules Going back to the original question and my apologies if this is obvious, but re: "I aliquot the compounds onto pedestal of sitting drop trays then wait for the DMSO to evaporate before adding my cryo-protectant solution and pre-grown protein crystals." I was wondering if there is any scope to use DMSO as the cryoprotectant then you wouldn't have to wait so long (if at all) for it to evaporate? I guess you have tested this. I remember the XChem people were very keen on having DMSO in everything about a decade ago. Best wishes, Jon Cooper (Emeritus at UCL) [email protected]<mailto:[email protected]> Erratum and other hopefully useful things: https://crxp.org.uk Sent from Proton Mail<https://proton.me/mail/home> for Android. -------- Original Message -------- On Friday, 05/22/26 at 08:02 Weiss, Manfred <[email protected]<mailto:[email protected]>> wrote: Dear Leon, you may want to check out the workflow at your F2X facility in Berlin. hz-b.de/F2X In particular, the paper in JoVE by Wollenhaupt et al. should be interesting for you (https://www.jove.com/de/t/62208/workflow-tools-for-crystallographic-fragment-screening-at-helmholtz) You are welcome to contact us offline, if you have more questions. Best wishes Manfred ________________________________ From: CCP4 bulletin board <[email protected]<mailto:[email protected]>> on behalf of Giang, Leon (MU-Student) <[email protected]<mailto:[email protected]>> Sent: Tuesday, May 12, 2026 6:16 PM To: [email protected]<mailto:[email protected]> Subject: [ccp4bb] Fragment soaking protein crystals with small molecules Hello everyone, I am looking for advice on my fragment soaking protocol. Recently, I obtained a crystal structure of a small molecule within my protein. These compounds are insoluble in aqueous solution so I've been preparing aliquots of the compound at known concentrations in 100% DMSO. I aliquot the compounds onto pedestal of sitting drop trays then wait for the DMSO to evaporate before adding my cryo-protectant solution and pre-grown protein crystals. I am trying to reproduce my results with different concentrations of compound but have to wait a long time for the DMSO to evaporate. I am looking for advice on how to speed up the DMSO evaporation. Thanks, Leon ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ________________________________ Helmholtz-Zentrum Berlin für Materialien und Energie GmbH Mitglied der Hermann von Helmholtz-Gemeinschaft Deutscher Forschungszentren e.V. Aufsichtsrat: Vorsitzender Michael Zimmermann, stv. Vorsitzende Dr. Jutta Koch-Unterseher Geschäftsführung: Prof. Dr. Bernd Rech, Saskia Vormfelde Sitz Berlin, AG Charlottenburg, HRB 5583 B Postadresse: Hahn-Meitner-Platz 1 14109 Berlin Deutschland Diese E-Mail kann vertrauliche und/oder rechtlich geschützte Informationen enthalten. Wenn Sie diese E-Mail irrtümlich erhalten haben, informieren Sie bitte sofort den*die Absender*in und vernichten Sie diese Mail. Das unerlaubte Kopieren, die Veröffentlichung sowie die unbefugte Weitergabe dieser Mail ist nicht gestattet. This email may contain confidential and/or proprietary information. If you have received this e-mail in error, please inform the sender immediately and destroy this e-mail. Unauthorized copying, publishing or distribution of this e-mail is not permitted. ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
