Going back to the original question and my apologies if this is obvious, but re: "I aliquot the compounds onto pedestal of sitting drop trays then wait for the DMSO to evaporate before adding my cryo-protectant solution and pre-grown protein crystals." I was wondering if there is any scope to use DMSO as the cryoprotectant then you wouldn't have to wait so long (if at all) for it to evaporate? I guess you have tested this. I remember the XChem people were very keen on having DMSO in everything about a decade ago.
Best wishes, Jon Cooper (Emeritus at UCL) [email protected] Erratum and other hopefully useful things: https://crxp.org.uk Sent from [Proton Mail](https://proton.me/mail/home) for Android. -------- Original Message -------- On Friday, 05/22/26 at 08:02 Weiss, Manfred <[email protected]> wrote: > Dear Leon, > > you may want to check out the workflow at your F2X facility in Berlin. > > hz-b.de/F2X > > In particular, the paper in JoVE by Wollenhaupt et al. should be interesting > > for you > (https://www.jove.com/de/t/62208/workflow-tools-for-crystallographic-fragment-screening-at-helmholtz) > > You are welcome to contact us offline, if you have more questions. > > Best wishes > Manfred > > --------------------------------------------------------------- > > From: CCP4 bulletin board <[email protected]> on behalf of Giang, Leon > (MU-Student) <[email protected]> > Sent: Tuesday, May 12, 2026 6:16 PM > To: [email protected] > Subject: [ccp4bb] Fragment soaking protein crystals with small molecules > > Hello everyone, > > I am looking for advice on my fragment soaking protocol. Recently, I obtained > a crystal structure of a small molecule within my protein. These compounds > are insoluble in aqueous solution so I've been preparing aliquots of the > compound at known concentrations in 100% DMSO. I aliquot the compounds onto > pedestal of sitting drop trays then wait for the DMSO to evaporate before > adding my cryo-protectant solution and pre-grown protein crystals. I am > trying to reproduce my results with different concentrations of compound but > have to wait a long time for the DMSO to evaporate. I am looking for advice > on how to speed up the DMSO evaporation. > > Thanks, > Leon > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > --------------------------------------------------------------- > > Helmholtz-Zentrum Berlin für Materialien und Energie GmbH > > Mitglied der Hermann von Helmholtz-Gemeinschaft Deutscher Forschungszentren > e.V. > > Aufsichtsrat: Vorsitzender Michael Zimmermann, stv. Vorsitzende Dr. Jutta > Koch-Unterseher > > Geschäftsführung: Prof. Dr. Bernd Rech, Saskia Vormfelde > > Sitz Berlin, AG Charlottenburg, HRB 5583 B > > Postadresse: > Hahn-Meitner-Platz 1 > 14109 Berlin > Deutschland > > Diese E-Mail kann vertrauliche und/oder rechtlich geschützte Informationen > enthalten. Wenn Sie diese E-Mail irrtümlich erhalten haben, informieren Sie > bitte sofort den*die Absender*in und vernichten Sie diese Mail. Das > unerlaubte Kopieren, die Veröffentlichung sowie die unbefugte Weitergabe > dieser Mail ist nicht gestattet. > This email may contain confidential and/or proprietary information. If you > have received this e-mail in error, please inform the sender immediately and > destroy this e-mail. Unauthorized copying, publishing or distribution of this > e-mail is not permitted. > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
