Herbert, Thank you very much for the reply. I guess I will have to see if I can find someone with a research company or university who would purchase it for me as I have not found anyone doing and research with FeLV cats at this time.
If I am thinking of the same study that had a 28% positive outcome, they said if you didn't count the very sick cats who died after only one or two treatments, it was 46%. What protocol did you use on your cats and for how long? Gary -----Original Message----- From: felvtalk-boun...@felineleukemia.org [mailto:felvtalk-boun...@felineleukemia.org] On Behalf Of hebert ferrarezzi Sent: Saturday, August 08, 2009 8:58 PM To: felvtalk@felineleukemia.org Subject: Re: [Felvtalk] Staph Protein A Dear Gary I have used Staphoccocal Protein-A, from Sigma Aldrich: Product: P6031 - 1MG, Protein A from Staphylococcus aureus; Soluble, essentially salt-free, lyophilized powder; or Product: P3838 - 1MG, Protein A from Staphylococcus aureus; Soluble, Cowan Strain, essentially salt-free, lyophilized powder. http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/productinformationsheet2 /p3838pis.Par.0001.File.tmp/p3838pis.pdf It is important to note that transgenic Protein A expressed in Escherichia colli, although having the same immunoglobulin binding properties, does not have the same superantigenic properties as the native one. The difference may be due to a bit of contamination with other superantigens from Staphlococcus aureus (perhaps with staph entherotoxin-A during product extraction and purification). So the transgenic version does not serve to our intent. This product is currently used as a chemical reagent, and not as a medication (except in experimental studies). You have to register before buying and affiliation to some university or research institute is wanted (maybe you can get an aid from another person). Although I am a researcher, I was not made any experiment with my FelV+ cats, so I cannot be sure if apparently hopeful outcomes were not merely by chance. I just think the subject merits more attention, considering the earlier surprising results obtained by scientists. I am also aware of a subsequent statistically controlled study carried on by Vet-researchers found no significant difference from the placebo group. But I am also aware that unknown variables, then unsuspected (such a little bit of contamination mentioned above, certainly a trait neither standard nor regularly desired by the fabricants), can account for different results obtained by different researchers in different circumstances. By the way, tree of my four FelV+ cats have already gone (crossed the bridge as you say), Pupa and Mãe victims of lymphoma, and Porchops due an idiopathic cystitis (apparently not felv-related). All the four got considerable improvement in most blood counts during and after treatment and never suffered from FelV-related opportunistic infections. Jorge, the one that remains in this world, is doing very fine, thanks, being asymptomatic up to now. Early experiments using protein A have reported that 28% of the cats respond positively to the treatment. I think the attempt was not deceiving yet. Best wishes for you all. Hebert _______________________________________________ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org
