My Annie was never positive until her owner went into hospice and she ws locked 
up in her trailer for 3 weeks, not knowing where Kathy was and only Kathy's 
sister coming in, changing the box and giving fresh water and food, no 
interaction.  Then when I said I would take her (Kathy said if no good home, 
pout to sleep) they put her in a cardboard box, brought to vet's office and I 
put her in my carrier and took her home to suddenly have to deal with 2 strange 
people and another cat.  Then she tested positive.  I think stress has a great 
deal to do with the onset of FELV.  She was 4 when I got her and is now going 
on 9, healthy as can be.  I keep my other cats vaccinated for FELV. 
 All are healthy except for Lil Bit who is now dealing with extreme bacteria 
infection in bladder, most likely kidneys.  Has nothing to do with Annie being 
FELV.  Lil Bit is now on the road to recovery I think.  Still wobbly, but her 
eyes are not dialated, she is eating (a little bit at a time because the first 
time she wanted food, gulped it down and up=chucked).  Now I let her eat a bit, 
make her rest a bit and on till she wants no more.  No more problems with 
up-chucking.  She is getting sub q fluids every other day and has been 
drinking.   I think part of her stumbling around in circles is she wants to get 
to the watr fountain.  When I let her out yesterday, she headed straight for it 
and began drinking.  Water in a bowl is ok, but fountain is better. 
---- Margo <> wrote: 
> Hi Shelley,
>         This is coming from a very disgruntled person so take it all with a 
> grain of salt:)
>         I have pretty much given up on test results as accomplishing 
> anything. They seem just about useless in the long run, from my experience. 
> For many years I ran a rescue in FL. I was fanatic about testing. Everyone 
> was Elisa tested on intake, and again in three months, if we still had them. 
> We offered follow-up testing to adopters. No positives were allowed to leave, 
> and every one tested negative (both Elisa and IFA) at 3 months. I figured I 
> was just very, very lucky.
>         Fast forward. Two years ago I re-tested my whole population. Elisa 
> negative. All of them. Just after that, I added a new cat. He was in 
> quarantine for three months. Clean Elisa at both times. In March, he tested 
> positive, after a very stressful bout of struvite stones. I was devastated. 
> Now what? Well, since then one more (of my original household) has tested +. 
> Another is likely. I'm not re-testing everyone. I'll test as they need to go 
> in for the complications.
>         Here's what "Shelter Medicine" (UC Davis) has to say about FeLV 
> testing;
> "What additional tests are available?
> Cats testing positive by the ELISA test on serum should ideally be retested 
> either using an ELISA test from a different manufacturer or by sending the 
> appropriate sample for an IFA test at a diagnostic laboratory. If both tests 
> are positive, the cat is very likely persistently infected. To be absolutely 
> certain, cats can be held and retested after 30 days if resources are 
> available and the cat can be appropriately housed and isolated (for the 
> protection of the cat and population; see below). Because the IFA is less 
> sensitive (more prone to false negatives) than the ELISA test, a negative IFA 
> result in a cat testing positive ELISA can not be taken as an indicator that 
> the cat is not infected. If the ELISA test is positive but the IFA results in 
> a negative result, both tests can be repeated in 30 days.  If this strategy 
> is used, a plan should be made at the outset and clearly communicated to 
> staff and foster parents regarding what will happen with cats that test 
> persistently positive
  after the 30 day hold. A PCR test can also be run to help resolve any 
conflicts in the tests. PCR is very sensitive, so a negative test result run by 
a reputable laboratory indicates infection is unlikely. However, because PCR 
testing is very sensitive to laboratory error, correct sample handling and 
laboratory quality are extremely important."
> Even so, the more I read, the less I trust ANY test to be accurate. Seems 
> that whatever the test, somehow there are ways it can prove to be inaccurate.
> So, I am at a loss. I have four kittens suitable for adoption. All tested 
> negative. All have been vaccinated against FeLV. And yet, they've lived 
> (completely seperately, physically) in a positive household. I don't want to 
> subject any adopter to the possibility of the heartbreak of FeLV. So they'll 
> stay. And I quit rescuing, fostering and re-hab. 
> Maybe I'll learn more (or someone else will) and change my mind.
> Margo
> -----Original Message-----
> >From: Shelley Theye <>
> >Sent: Aug 14, 2013 9:57 AM
> >To:
> >Subject: [Felvtalk] IFA tests/PCR tests
> >
> >Hi,
> >
> >I want to bring Leo back in to get the IFA test.  I was reading about the 
> >company that developed
> >the IFA test,  the National Veterinary Lab.  Are they the company that most 
> >folks use or can 
> >my vet send to any lab, like Antech, etc.  Is one lab considered more 
> >accurate than another?
> >
> >Are PCR tests done much? I think in England they are used more than the IFA, 
> >at least I recall
> >reading that a while back on a website.
> >
> >Thanks so much for any advice,
> >Shelley
> >
> >
> >
> >_______________________________________________
> >Felvtalk mailing list
> >
> >
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