Also, I'm working under John Kelly and Lena Hileman at KU. We, along with our collaborators at Duke, would get a lot of use out of an expected transcriptome that we could blast illumine data against. If we sent you our genome in .fasta format along with the .gff expected transcript file is their anyway you could combine these into a database we could align against?
-Jack On Nov 2, 2011, at 12:03 PM, Jack Colicchio wrote: > Hey, > > Jack Colicchio here, a PhD. student at KU. I am about to get an illumina > Next gen transcriptome data setthat I would like to align and quantify > against a list of expected transcripts from Mimulus guttatus. The expected > transcripts are in .gff format, and I was wondering how I could get that file > uploaded to you're website to allow me to align my transcriptome against. I > successfully uploaded the .GFF file, and can view it on your site, but do not > know how I could blast my .fastq data from illumine against this file. > > Thanks, > Jack ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
